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nrnA [2019-07-09 09:18:59]
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nrnA [2019-07-09 09:18:59]

oligoribonuclease (nanoRNase), 3,5-bisphosphate nucleotidase, degradation of pGpG
locus
BSU29250
pI
4.67
mw
34.93 kDa
protein length
313 aa Sequence Blast
gene length
942 bp Sequence Blast
function
degradation of RNA oligonucleotides
product
oligoribonuclease (nanoRNase), 3,5-bisphosphate nucleotidase
essential
no
ec
3.1.3.7
synonyms
ytqI
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.1|Exoribonucleases]

    • Gene

    Coordinates
    2,995,908 2,996,849

    Phenotypes of a mutant

  • The mutant requires cysteine [Pubmed|17586819]
  • inactivation of ''[gene|4C630B61249F8F2369E9BFE8A49885D3225DE7B6|nrnA]'' reduces sporulation efficiency to 5% that of wild type cells; delayed entry into sporulation, reduced SigmaG activity, and production of small forespores [Pubmed|26735940]

    • The protein

    Catalyzed reaction/ biological activity

  • oligoribonuclease (nanoRNAse), 3',5'-bisphosphate nucleotidase [Pubmed|17586819]
  • hydrolyzes oligo(deoxy)ribonucleotides in a 5' 3' direction [Pubmed|28894100,21087930]
  • hydrolyzes nano-RNAs in a 3' 5' direction [Pubmed|28894100]
  • degradation of pGpG derived from c-di-GMP [pubmed|30249708]

    • [SW|Domains]

  • [SW|DHH-DHHA1 domain] [pubmed|28894100]

    • [SW|Cofactors]

  • Mn2+ [pubmed|28894100]

    • Structure

  • [PDB|5J21] [pubmed|28894100]

    • [SW|Localization]

  • Cytoplasm (Homogeneous) [Pubmed|16479537]

    • Expression and Regulation

    Operons

    genes
    [gene|4C630B61249F8F2369E9BFE8A49885D3225DE7B6|nrnA]
    description
    [Pubmed|9387221]
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    Biological materials

    Mutant

  • MGNA-A151 (ytqI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/151 NBRP B. subtilis, Japan]
  • GP2155 (''[gene|4C630B61249F8F2369E9BFE8A49885D3225DE7B6|nrnA]''::''aphA3''), available in [SW|Jörg Stülke]'s lab
  • BKE29250 ([gene|4C630B61249F8F2369E9BFE8A49885D3225DE7B6|nrnA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGATACTCCTTTATTG, downstream forward: _UP4_GAGTAGAGGGGAGACCCTCT
  • BKK29250 ([gene|4C630B61249F8F2369E9BFE8A49885D3225DE7B6|nrnA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGATACTCCTTTATTG, downstream forward: _UP4_GAGTAGAGGGGAGACCCTCT

    • References

    Reviews

  • 29458847

    • Original publications

  • 17586819,9387221,16479537,19429620,19553197,21087930,22102036,26691161,21768284,24013631,26735940,27834680,28894100,30249708